We employed emerging smartphone-based location data and produced daily human mobility measurements using Nigeria as an application site. A data-driven analytical framework was developed for rigorously producing such measures using proven location intelligence and data-mining algorithms. Our study demonstrates the framework at the beginning of the SARS-CoV-2 pandemic and successfully quantifies human mobility patterns and trends in response to the unprecedented public health event. Another highlight of the paper is the assessment of the effectiveness of mobility-restricting policies as key lessons learned from the pandemic. We found that travel bans and federal lockdown policies failed to restrict trip-making behaviour, but had a significant impact on distance travelled. This paper contributes a first attempt to quantify daily human travel behaviour, such as trip-making behaviour and travelling distances, and how mobility-restricting policies took effect in sub-Saharan Africa during the pandemic. This study has the potential to enable a wide spectrum of quantitative studies on human mobility and health in sub-Saharan Africa using well-controlled, publicly available large data sets. SIGNIFICANCE: • The mobility measurements in this study are new and have filled a major data gap in understanding the change in travel behaviour during the SARS-CoV-2 pandemic in Nigeria. These measurements are derived from high-quality data samples by state-of-the-art data-driven methodologies and could be further adopted by other quantitative research related to human mobility. • Additionally, this study evaluates the impact of mobility-restricting policies and the heterogeneous effects of socio-economic and socio-demographic factors by a time-dependent random effect model on human mobility. The quantitative model provides a decision-making basis for the Nigerian government to provide travel-related guidance and make decisions in future public health events.

BACKGROUND: HIV infection causes immune dysregulation affecting T-cell and monocyte function, which may alter coronavirus disease 2019 (COVID-19) pathophysiology. OBJECTIVES: We investigated the associations among clinical phenotypes, laboratory biomarkers, and hospitalisation outcomes in a cohort of people hospitalised with COVID-19 in a high HIV prevalence area. METHOD: We conducted a prospective observational cohort study in Tshwane, South Africa. Respiratory disease severity was quantified using the respiratory oxygenation score. Analysed biomarkers included inflammatory and coagulation biomarkers, CD4 T-cell counts, and HIV-1 viral loads (HIVVL). RESULTS: The analysis included 558 patients, of whom 21.7% died during admission. The mean age was 54 years. A total of 82 participants were HIV-positive. People living with HIV (PLWH) were younger (mean age 46 years) than HIV-negative people; most were on antiretroviral treatment with a suppressed HIVVL (72%) and the median CD4 count was 159 (interquartile range: 66-397) cells/µL. After adjusting for age, HIV was not associated with increased risk of mortality during hospitalisation (age-adjusted hazard ratio = 1.1, 95% confidence interval: 0.6-2.0). Inflammatory biomarker levels were similar in PLWH and HIV-negative patients. Detectable HIVVL was associated with less severe respiratory disease. In PLWH, mortality was associated with higher levels of inflammatory biomarkers. Opportunistic infections, and other risk factors for severe COVID-19, were common in PLWH who died. CONCLUSION: PLWH were not at increased risk of mortality and those with detectable HIVVL had less severe respiratory disease than those with suppressed HIVVL. WHAT THIS STUDY ADDS: This study advances our understanding of severe COVID-19 in PLWH.

ABSTRACT Little is known about how Brazilian dentists’ treatment decisions for proximal carious lesions are compared to current evidence-based recommendations, so better understanding is needed to close any potential evidence-practice gap. Objectives: This cross-sectional study aimed to quantify the evidence-practice gap about proximal carious lesions treatment and identify dentist factors associated with this evidence-practice gap. Methods: Brazilian dentists (n=214) from Araraquara, São Paulo State, “completed a questionnaire about their dentist and practice characteristics and a translated version of the “Assessment of Caries Diagnosis and Caries Treatment” from the U.S. National Dental Practice-Based Research Network. Five radiographic images of proximal carious lesions in low-risk and high-risk patient scenarios were used. Associations between treatment recommendations and lesion, dentist, and practice characteristics were tested for statistical significance (p<0.05). Results: Lesions confined to the enamel would be restored by 35% and 71% of dentists in the low-risk and high-risk patient scenarios, respectively, suggesting a substantial evidence-practice gap given that surgical intervention of enamel lesions is not consistent with current evidence. The lesion depth threshold to recommend a permanent restoration differed between the low-risk and high-risk patient scenarios (p<0.001). Specific dentist/practice characteristics (dentist gender, graduate of a public dental school, postgraduate training, use of caries risk assessment) were significantly associated with the evidence-practice gap, but the magnitude of these differences was not major Conclusion: A substantial evidence-practice gap in treatment of proximal carious lesions was found for the sample overall, even when clinical scenarios presented low-risk patients. Global strategies are needed to close this substantial evidence-practice gap.

RESUMO Pouco se sabe se as decisões de tratamento dos dentistas brasileiros para lesões cariosas proximais são comparadas às recomendações atuais baseadas em evidências, portanto, é necessário um melhor entendimento para fechar qualquer potencial lacuna entre a evidência e a prática. Purpose: Este estudo transversal teve como objetivo quantificar a lacuna entre a evidência e a prática na decisão de tratamento das lesões cariosas proximais e identificar os fatores associados a essa lacuna entre a evidência e a prática. Methods: Cirurgiões-dentistas brasileiros (n=214) de Araraquara, Estado de São Paulo, preencheram um questionário sobre suas características odontológicas e clínicas e uma versão traduzida do “Assessment of Caries Diagnosis and Caries Treatment” da U.S. National Dental Practice-Based Research Network. Cinco imagens radiográficas de lesões cariosas proximais em cenários de pacientes de baixo risco e alto risco foram usadas. Associações entre recomendações de tratamento e lesão, dentista e características da prática foram testadas (p<0,05). Results: As lesões confinadas ao esmalte seriam restauradas por 35% e 71% dos dentistas nos cenários de pacientes de baixo risco e alto risco, respectivamente, sugerindo uma lacuna entre a evidência e a prática substancial, dado que a intervenção cirúrgica das lesões do esmalte não é consistente com as evidências atuais. O limiar de profundidade da lesão para recomendar uma restauração permanente diferiu entre os cenários de pacientes de baixo risco e alto risco (p<0,001). Características específicas do dentista/prática (sexo do dentista, graduado em uma faculdade pública, pós-graduação, uso da avaliação de risco de cárie) foram significativamente associadas à lacuna entre a evidência e a prática, mas a magnitude dessas diferenças não foi importante. Conclusions: uma lacuna entre a evidência e a prática substancial na decisão de tratamento de lesões cariosas proximais foi encontrada para a amostra como um todo, mesmo quando os cenários clínicos apresentavam pacientes de baixo risco. Estratégias globais são necessárias para fechar essa lacuna entre a evidência e a prática.

ABSTRACT The objective of this study was to evaluate the effect of intronic single nucleotide polymorphisms (SNP) on temperament traits in a Brahman cattle population. The SNP located in CACNG4, EXOC4, NRXN3, and SLC9A4 candidate genes were genotyped in 250 animals with temperament records of exit velocity, pen score, and temperament score. Rs3423464051:G>A in the CACNG4 gene was associated with exit velocity and temperament score. An in silico analysis of the five intronic SNP showed that alternative alleles of CACNG4-rs3423464051, EXOC4-rs109393235, and SLC9A4-rs109722627 SNP could alter branch point sites during splicing, while a protein–protein interaction network analysis demonstrated a GRIA2 gene-mediated interaction between CACNG4 and NRXN3. The present results support previously reported evidence regarding bovine temperament-related candidate genes, particularly CACNG4, which is a confirmed candidate gene in need of more detailed analyses to reveal its role in temperament-related traits.

Resumo Dentre os muitos relatos de “populismo de direita”, uns enfatizam a desigualdade de renda, outros a cultura, e outros ainda as especificidades de um dado sistema político. Este artigo dá um passo atrás para desenvolver uma visão estrutural de longo prazo. A tese principal propõe interpretar o cenário mundial enquanto crise da democracia capitalista. Os estilos políticos específicos e trajetos casuais que levaram homens como Trump, Bolsonaro, Duterte e Modi ao poder são difíceis de pontuar. O que nos parece óbvio é o crescente desprendimento de grande parte da população das formas democráticas liberais, cuja causa subjacente é o deslindamento da “base material de consentimento” que marcou as democracias capitalistas no pós-guerra. Não por acaso, este deslindamento tem mostrado maior progresso precisamente na periferia e semiperiferia. Esses países têm se mostrado inovadores políticos frente ao capitalismo mais consolidado do centro. Sustento essa argumentação por meio de uma breve análise da ascensão de Berlusconi na Itália, considerado o “canário na mina” da atual onda de figuras bonapartistas, e Bolsonaro, o mais importante exemplar do tipo atualmente.

Abstract There are many accounts of “right wing populism”. Some emphasize income inequality, some culture, some the specifics of particular political systems. This paper takes a step back, and provides a longer term structural view. Its central contention is that what is happening in the world today is a structural crisis of capitalist democracy. The specific political styles and causal pathways that brought men like, Trump, Bolsonaro, Duterte, and Modi to power are hard to specify. What is blindingly obvious is the increasing detachment of large parts of the population from liberal democratic forms. The underlying cause of this detachment is the unravelling of the “material basis of consent” that had marked capitalist democracies in the post-war period. Unsurprisingly this unravelling has progressed the most in precisely the periphery of the core, and the semi-periphery. It is these countries that have become the political path-breakers for the more consolidated capitalism’s of the core. I sustain these claims in what follows through a brief comparative analysis of the rise of Berlusconi in Italy who was the “canary in the coal mine” of the current wave of Bonapartist figures, and Bolsonaro who is the most important current exemplar of the type.

Abstract Mycotoxins are secondary metabolites produced by some filamentous fungi, which can cause toxic effects in humans and animals. The purpose of the study was to report the influence of postharvest conditions of grains and animal feed on the occurrence of mycotoxins in milk and dairy products which are widely consumed worldwide and have several health benefits for consumers. Among the most toxic mycotoxins are aflatoxins (AFs), with AFB1 being the most toxic and present in grains and cereals used in the feeding of dairy cows. After ingestion, AFB1 is converted to AFM1, which is excreted in milk, being a source of direct contamination to consumers of this product and dairy products. Thus, knowledge about this substance and the care that can be taken to reduce the consumption of contaminated food is a matter of great importance concerning public health and food safety. This review reports that the temperature and humidity at which the grains are stored, are the main conditions that can be controlled during storage, aiming to reduce the growth of fungi and the production of mycotoxins. This work reinforces that the continuous control of mycotoxins in milk and dairy products is of vital importance to obtain a safe product. Besides, strategies to mitigate the development of fungal contamination have been carefully revised to prevent the formation of these toxic substances.

Abstract Data on dental practices related to caries risk assessment (CRA) are scarce among Brazilian dentists. This study aimed to determine the prevalence of CRA use by dentists and factors associated with its use, as well as to quantify dentists’ ratings of the importance of specific factors when treatment planning. Dentists registered at the Regional Council of Dentistry of São Paulo State – Araraquara region were sent two paper questionnaires that comprised: a) characteristics of dentists themselves, their practices, and their patients; and b) the translated version of the “Assessment of Caries Diagnosis and Caries Treatment” Questionnaire from the U.S. National Dental Practice-Based Research Network. Participants were 206 dentists who currently practiced in Araraquara and treated dental caries. Descriptive statistics and multiple logistic regression analyses were used for data analysis. Thirty-six percent of the dentists reported they perform CRA and, among them, 36% indicated they record the assessment on a special form that is kept in the patient chart. More years since dental school graduation (OR = 1.1, p = 0.002) and holding an advanced academic degree (OR=2.6, p=0.005) were associated with a higher likelihood of performing CRA, whereas exclusively using a private practice model (OR = 0.5, p = 0.016) was associated with a lower likelihood of performing CRA. The current oral hygiene and commitment to return for follow-up were the most important risk factors for treatment planning. In conclusion, CRA was not a routine procedure in daily practice among the majority of participating dentists. Specific demographic, practice and academic education characteristics were associated with performing CRA.

ABSTRACT Objective: To describe the dental practice patterns related to caries prevention in children aged 6-18 years and associated factors. Material and Methods: Dentists (n=162) from Araraquara, Brazil, completed two paper questionnaires: (1) one about characteristics of their practice and their patient population; and (2) a translated version of the “Assessment of Caries Diagnosis and Caries Treatment” Questionnaire from the National Dental Practice-Based Research Network. Regression analyses were used for data analysis (p<0.05). Results: Dentists reported using in-office fluoride (IOF) and dental sealants (DS) in 74.2% and 45.1% of their pediatric patients, respectively. Regression analysis showed that female dentists (p=0.035 for DS; p=0.044 for IOF; p=0.011 for non-prescription fluoride rinse), those with advanced degrees (p=0.032 for prescription of fluoride), those who graduated from a private dental school (p=0.018 for chlorhexidine rinse), those who provided caries prevention regimens (p<0.001 for DS; p=0.004 for IOF; p=0.013 for non-prescription fluoride rinse), those with a greater percentage of patients interested in a caries prevention regimen (p=0.007 for non-prescription fluoride rinse), those working in a private practice model (p=0.047 for prescription of fluoride) were more likely to recommend some type of preventive methods to their pediatric patients. Conclusion: Dentists reported recommending IOF to most of their pediatric patients. Certain dentists’, practices’, and patients’ characteristics were associated to some caries prevention regimens recommended by dentists.

Domestic dogs (Canis familiaris) may serve as a reservoir or a sentinel for infectious disease pathogens that can affect human and wildlife health. To understand the role of tick-borne diseases in rural and lesser developed regions, we investigated the prevalence of several tick-borne pathogens in communal dogs of Zimbabwe. Blood samples from 225 dogs in northwest Zimbabwe were assessed by serology for Ehrlichia canis, Anaplasma phagocytophilum and Borrelia burgdorferi, and 241 samples were assessed by polymerase chain reaction (PCR) for Ehrlichia. There was a high seroprevalence (73%) of E. canis-specific antibodies in domestic dogs in northwest Zimbabwe, but follow up analyses via PCR and genetic sequencing indicated only 7.5% of the canines were actively infected with the organism. Whilst indicating that an organism serologically related to E. canis is likely present in the region, this data also shows that the organism is currently present in a relative minority of the domestic dogs in the region. Its presence as evidenced by both serologic and PCR analysis is significant because of the 'one health' paradigm, where humans and wildlife may be affected by the exposure to this pathogen in domestic dogs.

Background: Area under the curve to minimum inhibitory concentration (AUC/MIC) has been recommended by the 2020 updated vancomycin guidelines for dosing vancomycin for both efficacy and safety. Previously, AUC/MIC has been cumbersome to calculate so surrogate trough concentrations of 15-20 mg/dL were utilized. However, trough-based dosing is not a sufficient surrogate as AUC/MIC targets of 400-600 can usually be reached without achieving troughs of 15-20 mg/dL. Targeting higher trough levels may also lead to adverse events including acute kidney injury (AKI) and nephrotoxicity. Objective: To compare the mean total first day vancomycin dose in traditional trough-based dosing versus dosing recommended by an AUC/MIC dosing program. Methods: Adult inpatients who received at least 24 hours of IV vancomycin treatment were included in this single-center, retrospective cohort study. The primaryendpoint was difference in mean total first day vancomycin dose in milligrams (mg) received between patients’ traditional trough-based dosing and recommended dose via AUC/MIC electronic dosing calculator. Patients served as their own control by analyzing both actual dose received and dose recommended by the electronic AUC/MIC program. Rates of vancomycin induced adverse events, including acute kidney injury, elevated steady-state trough concentrations, and Red Man’s syndrome were also compared between patients who received doses consistent with the AUC/MIC dosing recommendation versus those who did not. Results: 264 patients were included in this study. Initial 24-hour vancomycin exposure was significantly lower with the recommended AUC/MIC dose versus the dose received (2380.7; SD 966.6 mg vs 2649.6; SD 831.8 mg, [95% CI 114.7:423.1] p=0.0007). Conclusions: Utilizing an electronic AUC/MIC vancomycin dosing calculator would result in lower total first day vancomycin doses.

This study intervenes in commonsensical ways of exploring and understanding educational assessment within an audit culture of measurement. Drawing on sociomateriality and methodological approaches associated with material culture and narrative inquiry, we curated an exhibition based on interviews with 13 informants and the things they brought to convey their assessment experiences. Based on analysis of their narratives, we clustered our findings and organised them into a gallery of two thing-centred installations: "Assessment and Tools," and "Assessment and Arts/Crafts." Curating the gallery led us to a creative way of articulating concerns about excessive assessment into a thing-interview protocol to be used in future inquiry, involving interviews with people and things. We showcase these installations, along with interview prompts, as an online exhibition. The aim is to continue the conversation on the future of assessment in connection to purposeful, equity-oriented education.

Abstract: Background and Aims: The phytochemistry study of Annonaceae has intensified in the last decades due to the discovery of secondary metabolites with antimicrobial activities. The inhibitory activities of extracts and compounds from these species for phytopathogens are less known. The antifungal activity of Sapranthus microcarpus was determined in vitro against six fungal pathogens of important crops. Methods: Hexane, methanol, and alkaloid extracts of the leaves and root and stem bark of S. microcarpus were evaluated to determine whether they could inhibit the mycelial growth of the phytopathogens. The combined activity of the most active extracts for each phytopathogen was also determined. The alkaloid liriodenine was isolated and identified as an antifungal principle. Key results: All extracts inhibited the growth of the six phytopathogens to some extent. The root bark alkaloid extract showed the highest activity. Fusarium oxysporum f. sp. lycopersici and Curvularia lunata were the most sensitive phytopathogens. Liriodenine was active against all the plant pathogens (minimum inhibitory concentrations of 125-500 nmol ml-1). Conclusions: Sapranthus microcarpus is a native natural resource with great phytochemical potential.

Resumen: Antecedentes y Objetivos: El estudio de la fitoquímica de Annonaceae se ha intensificado en las últimas décadas debido al descubrimiento de metabolitos secundarios con actividades antimicrobianas. Las actividades inhibidoras de extractos y compuestos de estas especies para fitopatógenos son menos conocidas. Se determinó la actividad antifúngica in vitro de Sapranthus microcarpus contra seis hongos fitopatógenos de cultivos importantes. Métodos: Se evaluaron extractos metanólicos, hexánicos y alcaloidales de las hojas, y corteza de tallos y raíces de S. microcarpus por el método de inhibición micelial. También se determinó la actividad combinada de los extractos más potentes contra cada fitopatógeno. Se aisló e identificó al alcaloide liriodenina como uno de los metabolitos antifúngicos. Resultados clave: Todos los extractos, en alguna medida, inhibieron el crecimiento de los seis fitopatógenos. Los extractos alcaloidales mostraron la mayor actividad. Fusarium oxysporum f. sp. lycopersici y Curvularia lunata fueron los fitopatógenos más sensibles. Liriodenina fue activo contra todos los fitopatógenos (a concentraciones inhibitorias mínimas de 125-500 nmol ml-1). Conclusiones: Sapranthus microcarpus es un recurso nativo con alto potencial fitoquímico.

Abstract Background: There remains variability in both practice and evidence related to optimal initial empiric dosing strategies for vancomycin. Objective: Our primary objective was to describe the percentage of obese patients receiving vancomycin doses consistent with nomogram recommendations achieving targeted initial steady-state serum vancomycin concentrations. Secondary objectives were to describe the primary endpoint in subgroups based on patient weight and estimated creatinine clearance, to describe the rate of supratherapeutic vancomycin accumulation following an initial therapeutic trough concentration, and to describe the rate of vancomycin-related adverse events. Methods: This single-center, IRB-approved, retrospective cohort included adult patients ≥ 100 kilograms total body weight with a body mass index (BMI) >30 kilograms/m2 who received a stable nomogram-based vancomycin regimen and had at least one steady-state vancomycin trough concentration. Data collected included vancomycin regimens and concentrations, vancomycin indication, serum creatinine, and vancomycin-related adverse events. Patients were divided into two cohorts by goal trough concentration: 10-15 mcg/mL and 15-20 mcg/mL. Results: Of 325 patients screened, 85 were included. Goal steady-state concentrations were reached in 42/85 (49.4%) of total patients. Conclusions: Achievement of initial steady-state vancomycin serum concentrations in the present study (approximately 50%) was consistent with the use of published vancomycin dosing nomograms.

BACKGROUND Leprosy is a chronic infectious disease caused by the obligate intracellular bacillus Mycobacterium leprae. Because leprosy diagnosis is complex and requires professional expertise, new tools and methodologies are needed to detect cases in early stages and prevent transmission. The M. leprae genome contains mce1A, which encodes a putative mammalian cell entry protein (Mce1A). We hypothesised that the presence of Mce1A on the cell surface could be detected by the host's immune system. OBJECTIVE The aim of this study was to evaluate antibody responses against the Mce1A protein in leprosy patients, household contacts of patients, and the general population to present an addition tool for leprosy diagnosis. METHODS A cross-sectional study involving 89 volunteers [55 leprosy cases, 12 household contacts (HHC) and 22 endemic controls (EC)] was conducted at Couto Maia Hospital, in Salvador, Bahia (BA), Brazil. RESULTS The median anti-Mce1A IgA was significantly higher in multibacillary (MB) and paucibacillary (PB) cases than in EC (p < 0.0001). A similar trend was observed in IgM levels, which were significantly higher in both MB (p < 0.0001) and PB (p = 0.0006) groups compared to in EC individuals. The greatest differences were observed for IgG class-specific antibodies against Mce1A. The median levels of MB and PB were significantly higher compared to both controls HHC and EC (MB or PB vs EC, MB vs HHC p < 0.0001; PB vs HHC, p = 0.0013). Among leprosy cases, IgG enzyme-linked immunosorbent assay sensitivity and specificity were 92.7% and 97.1%, respectively. IgG positivity was confirmed in 92.1% and 94.1% of MB and PB patients, respectively. CONCLUSION This novel diagnostic approach presents an easy, non-invasive, and inexpensive method for leprosy screening, which may be applicable in endemic areas.